Cellular-resolution high-throughput imaging over an extended volumetric coverage in scattering biological media is desirable for many biological studies. Such capability enables the study of collective (emergent) cellular dynamics in both in vitro engineered biological systems and in vivo models of diseases. However, current optical microscopy techniques suffer from the detrimental effects of optical aberrations and ‘multiple scattering (MS)’ in biological media. Our lab has developed computed optical coherence microscopy (OCM) methods to enable geometrically accurate (distortion-free) volumetric OCM reconstructions that are computationally corrected for defocus and optical aberrations. Combining both hardware and computational approaches, we introduced hybrid adaptive optics (hyAO) to overcome the effects of MS in biological media, and increase the volumetric throughput of OCM. Leveraging computed OCM, we are also developing a multimodal hyAO approach to enable faster and deeper volumetric OCM and three-photon microscopy.
See our computed OCM and hyAO poster here.
Computed OCM papers
- Wu, M., Small, D.M., Nishimura, N., Adie, S.G. “Computed optical coherence microscopy of mouse brain ex vivo,” J. Biomed. Opt. 24(11) 116002
Hybrid AO papers
- Liu, S., Lamont, M.R.E., Mulligan, J.A., Adie, S.G., “Aberration-diverse optical coherence tomography for suppression of multiple scattering and speckle,” Biomed. Opt. Express 9, 4919-4935 (2018)
- Liu, S., Mulligan, J.A., Adie, S.G. “Volumetric optical coherence microscopy with a high space-bandwidth-time product enabled by hybrid adaptive optics,” Biomed. Opt. Express 9, 3137-3152 (2018)