Photo by Dave Burbank
Photo by Dave Burbank
Welcome to the Adie Lab
Our research focuses on the development and application of novel optical imaging methods that can provide access to new information for basic science investigations and clinical applications. Our main imaging modality is optical coherence tomography (OCT), which is an optical version of ultrasound that can enable cellular-scale, label-free imaging of tissue structure and function in vivo.
Computational imaging and adaptive optics. We are developing novel approaches to acquire and reconstruct OCT images, in order to extend the speed and imaging depth limits of optical microscopy. We introduced ‘hybrid adaptive optics’ (hyAO), an imaging approach that integrates computational and hardware adaptive optics, and have used it to enhance volumetric throughput of cellular-resolution OCT, and to suppress the effects of multiple scattering (a key factor limiting imaging depth in optical microscopy). Through collaborative efforts, we are extending hyAO to multimodal imaging, in particular to help adaptive-optics three-photon microscopy (AO-3PM) image faster and deeper.
Optical coherence elastography (OCE) and traction force microscopy (TFM). We are developing new approaches for 4D imaging of biophysical cell-extracellular matrix (ECM) interactions, including cellular traction forces and ECM mechanical properties. These efforts take advantage of the label-free, interferometric, and deep imaging capabilities of OCT, and make use of localized ‘pushing/palpation’ forces of photons or focused ultrasound. Our ‘home-built’ imaging systems and computational methods uniquely support long-term imaging of dynamic cell-ECM interactions during collective cell invasion in tumor spheroid collagen cell cultures. Our work on developing OCE methods based on ‘ultrasound pushing/palpation’ is to enable mechanical microscopy of the tumor microenvironment in vivo.
Basic science and clinical applications. We leverage the advantages of our custom-designed and built OCT and multimodal imaging systems to better understand the development and progression of disease. Of particular interest is the application of our new imaging techniques to the field of Cell Mechanics and Mechanobiology. We also aim to translate our new imaging capabilities and the discoveries that they may enable to impact the diagnosis or treatment of disease. These efforts benefit greatly from multidisciplinary collaborations with other researchers at Cornell (main Ithaca campus as well as Weill Cornell Medicine in New York City).