Hybrid adaptive optics for multimodal microscopy

Hybrid adaptive optics (hyAO), which harnesses the benefits of both hardware and computational adaptive optics (CAO), can be applied to multimodal imaging by integrating OCT with other imaging modalities.

In particular, we seek to leverage the ultra-deep aberration sensing capabilities of long-wavelength optical coherence microscopy to rapidly sense wavefront aberrations deep in the mouse brain, and then to leverage the close connection between hardware AO and CAO to enable adaptive optics three-photon microscopy (AO-3PM) image faster and deeper in the mouse and adult zebrafish brains. This research area is a collaboration with the Xu Research Group.

For further information see Liu et al., Proc. SPIE 11630, 2021 for a presentation on wavefront sensing and correction in mouse brain by CAO-OCM, and Yang et al., Proc. SPIE 1164817, 2021 for recent results from our multimodal microscopy system that combines long-wavelength OCM, 3PM and third-harmonic generation (THG) microscopy.

Relevant publications

  1. Liu, S., Xia F., Yang X., Wu M., Bizimana L., Xu C., Adie S.G., “Closed loop wavefront sensing and correction in mouse brain enabled by computed optical coherence microscopy”, Proc. SPIE, 1163007 (2021) https://doi.org/10.1117/12.2583924.
  2. Yang X., Liu S, Xia F., Wu M., Adie A.G., Xu C., “Simultaneous multimodal optical coherence and three-photon microscopy of the mouse brain in the 1700 nm optical window in vivo“, Proc. SPIE, Vol. 11648, Multiphoton Microscopy in the Biomedical Sciences XXI; 1164817 (2021) https://doi.org/10.1117/12.2582750.