Cellular traction forces (CTFs) play a role in many physiological and disease processes, including cell migration, cancer metastasis, stem cell differentiation, wound healing, and synapse formation. Traction force microscopy (TFM) is a family of optical techniques used to quantify CTF. Typical methods for 3D TFM are based on confocal fluorescence microscopy, and can suffer from limited penetration depth, slow acquisition speeds, and photobleaching/phototoxicity concerns. Our lab developed traction force optical coherence microscopy (TF-OCM) to overcome these challenges and enable 4D TFM for the study of both single cells and multi-cellular constructs in scattering media.
See our poster on TF-OCM here.
- Mulligan, J.A., Feng, X. & Adie, S.G. “Quantitative reconstruction of time-varying 3D cell forces with traction force optical coherence microscopy,” Sci. Rep. 9, 4086 (2019)
- Mulligan, J.A., Bordeleau, F., Reinhart-King, C.A., Adie, S.G. “Measurement of dynamic cell-induced 3D displacement fields in vitro for traction force optical coherence microscopy,” Biomed. Opt. Express 8, 1152-1171 (2017)