In this paper we demonstrate quantitative TF-OCM for reconstruction of 3D cell forces with minute-scale temporal resolution. Due to the ability of computational OCM to perform large-scale volumetric and label-free imaging in scattering media, TF-OCM may prove advantageous for the study of the force dynamics of large cell collectives in the burgeoning field of mechanobiology.
Lin obtained his B.Sc from Nankai University in 2013 and his Ph.D from The Hong Kong Polytechnic University in 2018, under the supervision of Prof. Wei Jin. During his Ph.D studies, he explored novel ultra-sensitive optical spectroscopy techniques, especially the microstructure-enhanced photothermal spectroscopy in photonic crystal fibres. His current research focuses on new approaches for photonic force optical coherence elastography, and quantitative investigations of mechanical properties for stem cell mechanobiology research.
Siyang’s recent hybrid adaptive optics paper published in Biomedical Optics Express has been highlighted as an Editor’s Pick.
Editor’s Picks serve to highlight articles with excellent scientific quality and are representative of the work taking place in a specific field.
Click on the link to go to the Cornell Chronicle’s article entitled New microscopy method could benefit study of migrating cancer cells.
In this paper we introduce a new ‘hybrid adaptive optics’ (hyAO) approach that synergistically combines hardware adaptive optics (HAO) and computational adaptive optics (CAO). We show that hyAO can significantly increase the throughput of large-volume, optical coherence microscopy, and use it to image cell population dynamics over a 1mm × 1mm × 1mm field-of-view with 2 μm isotropic spatial resolution and 3-minute temporal resolution.